HIV/AIDS Flashcards by Hanna B Brainscape

Human Retroviruses – Elisa Vicenzi • Guido Poli – Bok

Can successfully amplify and reliably report results with viral loads ≥1,000 copies/mL. Coreceptor tropism testing should be performed prior to initiation of therapy with CCR5 inhibitors. HIV‐1 tropism can be assessed with either phenotypic or genotypic methods. The Trofile assay (Monogram Biosciences, South San Francisco, CA) generates pseudovirions from the patient‐derived envelope ( env ) gene 8 . 6 6 0///481 Research Article Open Access J o u r n a l ¢ o f-A I D S & Cli n i c a l R e s e a r c h I 2111 9 1044,5002 1/05 696 0/-3061.1044,5002-0///481 esearch Co-Receptor Tropism Determined by Genotypic Assay in HIV-1 In research settings, co-receptor tropism testing has shown that approximately 80% of antiretroviral-naïve patients and approximately 60% of antiretroviral-experienced patients harbor the R5 virus, but there are little data documenting use of this assay in clinical practice. 2–4 The Trofile-ES assay was not used in the MOTIVATE or A4001029 studies.

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HIV-1 coreceptor tropism testing is used to evaluate eligibility for CCR5 antagonist therapy. However, HIV-1 RNA-based tests are not suitable for virologically suppressed patients, therefore the use of proviral DNA tropism testing has been investigated. We describe a novel proviral DNA-based genotypic tropism assay and compare its performance to Trofile Co-Receptor Tropism Assay Useful For Suggests clinical disorders or settings where the test may be helpful. To determine the co-receptor tropism Method Name A short description of the method used to perform the test. CD4 cell culture assay for phenotypic NY State Available Indicates A co-receptor tropism assay should be performed whenever the use of a CCR5 co-receptor antagonist is being considered (AI). Co-receptor tropism testing is recommended for patients who exhibit virologic failure on a CCR5 antagonist (BIII) . The assay's purpose is to identify the tropism of an individual patient's HIV strain – R5, X4, or a combination of these known as dual/mixed (D/M).

Klinisk prövning på HIV-infektioner: Placebo, Aprepitant

Co-receptor tropism testing is recommended for patients who exhibit virologic failure on a CCR5 antagonist (BIII) . The assay's purpose is to identify the tropism of an individual patient's HIV strain – R5, X4, or a combination of these known as dual/mixed (D/M).

Dynamics of HIV coreceptors and their utilization by plasma

recommend that a co-receptor tropism assay be performed whenever the use of a CCR5 inhibitor is being considered (AI). Co-receptor tropism testing is also recommended for patients who exhibit virologic failure on a CCR5 inhibitor (BIII). A phenotypic tropism assay (e.g., Trofile) is preferred to determine HIV-1 co-receptor usage (AI). Co-receptor tropism is defined as an interaction of a virus with a specific co-receptor on the target cell. To gain entry into CD4+ cells, HIV must bind to the cell surface CD4 receptor and to one of two co-receptors, CCR5 or CXCR4. Trofile DNA uses the complete gp160 coding region of the HIV-1 envelope protein ensuring that all of the determinants In this study, we developed a new phenotypic HIV-1 tropism assay (TROCAI [tropism coreceptor assay information]) based on the production of viral isolates from patients and multiple rounds of infection of indicator cells, modifying a technique previously reported by our group .

Can successfully amplify and reliably report results with viral loads ≥1,000 copies/mL. Consider Trofile Select when tropism testing is desired and viral load is unknown. Allows for ordering of Human Immunodeficiency Virus 1 (HIV-1) coreceptor tropism status to be completed using the appropriate assay, Trofile® or Trofile® DNA, based on the results of an initial prescreening viral assessment by the laboratory. The coreceptor • A co-receptor tropism assay should be performed whenever the use of a CCR5 co-receptor antagonist is being considered (AI). • Co-receptor tropism testing is recommended for patients who exhibit virologic failure on a CCR5 antagonist (BIII).
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Se hela listan på prn.org Trofile Coreceptor Tropism Assay: Is 100% sensitive at detecting 3% CR4-using minor variant; Uses the complete gp160 coding region of the HIV-1 envelope protein to ensure that all determinants of tropism are tested; Can successfully amplify and reliably report results with viral loads greater than or equal to 1,000 copies/mL Coreceptor usage was determined by viral tropism assays: 290 (68.9%) patients had CCR5-tropic HIV-1 virus, and 131 (31.1%) had dual-tropic/mixed or CXCR4 virus variants.

It must first bind CD4 and then, secondly, a chemokine receptor. Tropism is a label given to the virus that describes which chemokine receptor the virus is using. Co-receptor tropism testing is recommended for patients who exhibit virologic failure on a CCR5 antagonist (BIII). A phenotypic tropism assay is preferred to determine HIV-1 co-receptor usage (AI).
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Information från Läkemedelsverket Nr 1, 2008 - Studylib

CD4 cell culture assay for phenotypic NY State Available Indicates • Co-receptor tropism testing is recommended for patients who exhibit virologic failure on a CCR5 antagonist (BIII). • A phenotypic tropism assay is preferred to determine HIV-1 co-receptor usage (AI). • A genotypic tropism assay should be considered as an alternative … 2015-02-01 Data on coreceptor tropism in non-B HIV-1 subtypes are limited. We studied coreceptor tropism in HIV-1 circulating in Thailand, where CRF01_AE predominates, using a genotypic assay.